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DFF40, NT (CAD I18, DNA Fragmentation Factor, Caspase Activated Deoxyribonuclease, CPAN)

DFF40, NT (CAD I18, DNA Fragmentation Factor, Caspase Activated Deoxyribonuclease, CPAN)

Cat no: D3224-33A

Supplier: United States Biological
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Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Death domain containing adapter molecules and members of the caspase family of proteases transduce cell death signals. These death signals cause the final degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was recently identified and designated Caspase Activated Deoxyribonuclease (CAD). Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunohistochemistry: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D3224-33A
Reactivities: Mouse, Rat
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Enari M. et al. Nature 391, 43 (1998). 2. Sakahira H. et al. Nature 391, 96 (1998). 3. Liu X. et al. Proc. Natl. Acad. Sci. USA 95, 8461 (1998). 4. Halenbeck R. et al. Curr. Biol. 8,537 (1998). 5. Liu, X. el al. Cell 89, 175 (1997).
Additional info: Recognizes mouse CAD. Species Crossreactivity: rat.

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