Home  >  Products  >  DFF45 (DNA Fragmentation Factor 45kD Subunit, DFF 45, DFF-45, A330085O09Rik, DFF 1, DFF1, DFF35, DNA Fragmentation Factor alpha Subunit, DFF alpha, DFFA, H13, Inhibitor of Caspase Activated DNase, Inhibitor of CAD, ICAD, ICAD L, ICAD S, MGC143066)
DFF45 (DNA Fragmentation Factor 45kD Subunit, DFF 45, DFF-45, A330085O09Rik, DFF 1, DFF1, DFF35, DNA Fragmentation Factor alpha Subunit, DFF alpha, DFFA, H13, Inhibitor of Caspase Activated DNase, Inhibitor of CAD, ICAD, ICAD L, ICAD S, MGC143066)

DFF45 (DNA Fragmentation Factor 45kD Subunit, DFF 45, DFF-45, A330085O09Rik, DFF 1, DFF1, DFF35, DNA Fragmentation Factor alpha Subunit, DFF alpha, DFFA, H13, Inhibitor of Caspase Activated DNase, Inhibitor of CAD, ICAD, ICAD L, ICAD S, MGC143066)

Cat no: D3224-10G

Supplier: United States Biological
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Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (1). Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase (2,3). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (2,3). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death (4). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000, Murine lung tissue lysate can be used as positive control and a 45 kDa band can be detected. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: D3224-10G
Reactivities: Mouse
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified immunoglobulin
Alternative names: RP23 121D17.3
References: 1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184 2. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50 3. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99 4. Wyllie A. Apoptosis. An endonuclease at last. Nature 1998;39120-21.
Additional info: Recognizes mouse ICAD.

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