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DGKB (Diacylglycerol Kinase beta, Diglyceride Kinase beta, DGK-beta, DAG Kinase beta, 90kD Diacylglycerol Kinase, DAGK2, KIAA0718)

Cat no: D3441-82B

DGKB (Diacylglycerol Kinase beta, Diglyceride Kinase beta, DGK-beta, DAG Kinase beta, 90kD Diacylglycerol Kinase, DAGK2, KIAA0718)

Diacylglycerol kinase beta (DGK-b) is an ~90kD kinase that phosphorylates the lipid diacylglycerol. It is expressed in the brain and colocalizes with D1 and D2 dopamine receptors. DGK-b promotes the formation and maturation of dendritic spines. It contains two EF-hand motifs (aa149-184, 194-229), two zinc finger regions (aa244-294, aa309-358), and a catalytic domain (aa434-568). Alternate splicing generates an additional isoform with a 31aa C-terminal deletion. Over aa 1-143, human DGK-b shares 97aa sequence identity with mouse and rat DGK-b.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3441-82B

Size

100ug

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human DGK-b. Does not react with recombinant human DGK-a, g, e, i, K, th, or z.

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