DHPR, alpha 2 (Dihydropyridine Receptor, CACNA1S)

Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling. The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.\n\nApplications: \nSuitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nImmunohistochemistry (Paraffin, frozen): 1:500. Staining of DHP in rabbit skeletal muscle results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines.\nImmunocytochemistry: 1:250 \nWestern Blot: 1:500. Detects a band of ~220kD (non-reducing conditions) or ~143kD (reducing conditions).\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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