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DHX29 (ATP-dependent RNA Helicase DHX29, DEAH Box Protein 29, Nucleic Acid Helicase DDXx, DDX29)

DHX29 (ATP-dependent RNA Helicase DHX29, DEAH Box Protein 29, Nucleic Acid Helicase DDXx, DDX29)

Cat no: D6510-40D

Supplier: United States Biological
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DHX29 is an ATP-dependent RNA helicase that belongs to the DEAD-box helicase family (DEAH subfamily). DHX29 contains one central helicase and one helicase at the carboxy-terminal domain (1). Its function has not been fully established but DHX29 was recently shown to facilitate translation initiation on mRNAs with structured 5' untranslated regions (2). DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. Hydrolysis of nucleotide triphosphates by DHX29 is strongly stimulated by 43S complexes and is required for DHX29 activity in promoting 48S complex formation (2). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D6510-40D
Reactivities: Human, Mouse, Non-Human Primate
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: SUpernatant
References: 1.de la Cruz, J. et al. (1999) Trends Biochem Sci 24, 192-8. 2.Pisareva, V.P. et al. (2008) Cell 135, 1237-50.
Additional info: Recognizes endogenous levels of total human DHX29. Species Crossreactivity: Monkey and mouse.

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