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Dicer (Endoribonuclease Dicer, Helicase with RNase Motif, Helicase MOI, DICER1, HERNA, KIAA0928)

Dicer (Endoribonuclease Dicer, Helicase with RNase Motif, Helicase MOI, DICER1, HERNA, KIAA0928)

Cat no: D3520-01R

Supplier: United States Biological
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Dicer is an RNase III-like enzyme which cleaves double-stranded RNA into 21-23 nt RNAs. These RNAs are referred to as siRNA (short interfering RNA). It is believed to be essential in siRNA-mediated gene suppression. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: ELISA: 1:200 Flow Cytometry (Intracellular): 1:100-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D3520-01R
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Flow Cytometry
Size: 100ul
Form: Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Serum.
References: 1. Matsuda,S., Ichigotani,Y., Okuda,T., Irimura,T., Nakatsugawa,S. and Hamaguchi,M. Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase Biochim. Biophys. Acta 1490 (1-2), 163-169 (2000) 2. Nagase,T., Ishikawa,K., Suyama,M., Kikuno,R., Hirosawa,M., Miyajima,N., Tanaka,A., Kotani,H., Nomura,N. and Ohara,O. Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro DNA Res. 6 (1), 63-70 (1999).
Additional info: Recognizes human Dicer.

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