Dithiothreitol (DTT)

Cat no: D8070

Supplier: United States Biological

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Dithiothreitol (DTT) is the common name for a small-molecule redox reagent known as Cleland's reagent. DTT's oxidized form is a disulfide-bonded 6-membered ring. Its name derives from the four-carbon sugar, threose. DTT has an epimeric ('sister') compound, dithioerythritol (DTE). DTT is an unusually strong reducing agent, owing to its high conformational propensity to form a six-membered ring with an internal disulfide bond. It has a redox potential of -0.33 V at pH 7. The reduction of a typical disulfide bond proceeds by two sequential thiol-disulfide exchange reactions and is illustrated below. The intermediate mixed-disulfide state is unstable (i.e., poorly populated) because the second thiol of DTT has a high propensity to close the ring, forming oxidized DTT and leaving behind a reduced disulfide bond. The reducing power of DTT is limited to pH values above ~7, since only the negatively charged thiolate form -S

Catalogue number:	D8070
Size:	10g
Form:	White crystalline powder
Purity:	(same/more than)99%
Alternative names:	1,4-Dithiothreitol
References:	US Biological application reference: Tavares, C.D.J. et al., (2012) Biochemistry DOI: 10.1021/bi201788e. 1. Merck Index, 11th Edition, 3382. 2. Cleland WW. (1964) "Dithiothreitol, A New Protective Reagent for SH Groups", Biochemistry, 3, 480-482. 3. Ruegg UT and Rudinger J. (1977) "Cleavage of disulfide bonds in proteins", Methods Enzymol., 47, 111.

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Dithiothreitol (DTT)

Cat no: D8070

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