DNA/RNA Damage Monoclonal Antibody (Clone 15A3)

Antigen: 8-hydroxy guanosine-BSA and casein conjugates . Host: mouse, clone 15A3 . Cross Reactivity: (+) 8-hydroxy-2-deoxy guanosine, 8-hydroxy guanine, and 8-hydroxy guanosine . Applications: ELISA, IAC, and IHC . Isotype: IgG2b . 8-hydroxy guanine, 8-hydroxy-2'-deoxy guanonsine, and 8-hydroxy guanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine, produced by RONS including hydroxyl radical peroxynitrite, induces G to T transversions, which is one of the most frequent somatic mutations. 8-hydroxy guanine DNA base damage arises from radical-induced hydroxylation and cleavage reactions of the purine ring. 8-hydroxy guanosine also induces a mutagenic transversion of G to T in DNA.

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SPECIFICATIONS

Catalog Number

10011446

Applications

ELISA, IHC

Hosts

Mouse

Form

100 microg

P Type

Antibodies

Weight

0

Antigen

8-hydroxy-guanosine-BSA and casein conjugates

Isotype

IgG2b

Storage Temp

-20

Shipping Temp

-20

Additional Info

DNA and RNA damage is due to enviromental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. Four main types of DNA are endogenous cellular processes oxidation, alkylation, hydrolysis, and of the mismatch bases. Oxidation of bases is caused by reaction with reactive oxygen and nitrogen species (RONS), which include nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide, and peroxynitrite. Numerous studies have shown that RONS causes a variety biomolecular modifications including DNA damage. 8-hydroxy guanine, 8-hydroxy-2