DnaK, Full Length, aa1-638, Recombinant, E. coli

DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. DnaK (amino acids 1-638) was amplified by PCR and cloned into an E. coli expression vector.\n\nMolecular Weight: \n69kD (638 amino acids)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D4015-17

Size

500ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 100mM sodium chloride, 5mM DTT, 10% glycerol.

Purity

(same/more than) 95% by SDS PAGE; Purified by using conventional chromatography techniques.

References

1. Bardwell & Craig, et al., Proc. Natl. Acad. Sci. 81: 848-852 (1984). 2. Zhu, et al., Science 272: 1606-1614 (1996). 3. Tanaka., N., et al.., PNAS 26(99): 15,398-15,403 (2002).