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DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like MGC1090)

DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like MGC1090)

Cat no: D3931-73L

Supplier: United States Biological
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Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue (1-6). Studies show that DNMT3L regulates the activity of DNMT3A and DNMT3B and stimulates their catalytic activities (7). DNMT3L has specifically been linked to the process of carcinogenesis, thru its role in nuclear programming (8). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot:1-10ug/ml Immunocytochemistry:0.1-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D3931-73L
Reactivities: Human, Mouse
Hosts: Mouse
Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH7.4, 50% glycerol and 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Bestor T., et al.(1988) J.Mol. Biol. 203:971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D. et al. (1992) Nucl. Acids Res. 20:2287-2291. 3. Xie S. et al. (1999) Gene 236:87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99:247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23:380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25(3):338-342. 7. Kareta MS., et al. (2006) J Biol Chem. 281(36):25893-25902. 8. Gokul G., Ramakrishna G., and Khosla S. (2009) Epigenetics. 4(5):322-329.
Additional info: Recognizes human DNMT3. No Crossreactivity: against DNMT3a or DNMT3B.

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