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DOCK2 (Dedicator of Cytokinesis Protein 2, KIAA0209)

Cat no: D8080-05M

DOCK2 (Dedicator of Cytokinesis Protein 2, KIAA0209)

DOCK2 (Dedicator of cytokinesis protein 2) is a member of the DOCK-A family, DOCK180 superfamily of proteins. Its predicted molecular weight is 210kD, although it may run approximately at 190kD in SDS-PAGE. It is found in hematopoietic cell types, and regulates the activity of the GTPases Rac-1 and -2. Human DOCK2 is 1830aa in length. It contains one SH3 domain (aa8-69), a DHR-1 region that localizes DOCK2 to the plasma membrane (aa420-662), and a DHR-2 domain that binds to nucleotide-free GTPases and shows GEF activity (aa1115-1621). There are two isoform variants, one that shows a 38aa substitution for Tyr1405, and a second that shows a start site at Met450 with accompanying deletions of aa462-494 and 630-766, and a 22aa substitution for aa934-1830. Over aa420-642, human DOCK2 shares 96% aa identity to mouse DOCK2.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot:1g/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D8080-05M

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum, Goat

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human DOCK2. Species sequence homology: mouse (96%).

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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