Tissue cDNA Panel (Matched Pairs), Human Primary and Matched Metastasis Tumor, Breast, BioGenomics(TM)

BioSelect(TM) Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.\n\nHuman cDNA matched pair products include: Primary Pair (PP), or Primary and Metastatic Pair (PM). PP consists of cDNAs isolated from primary tumor and its adjacent normal tissue; PM consists of cDNAs from primary tumor and corresponding metastatic tumor. cDNAs in each pair are prepared from the same donor. This product line is designed for identifying tumor-specific genes and tumor. \n\nThe cDNA panel is comprised of 2 vials each containing 10ul of PCR Ready First Strand cDNA. All cDNAs in a panel are made under optimal and consistent conditions, which ensure that representation is maintained from total RNA to cDNA. The First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented normal and tumor tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. \n\nContents:\nPrimary Tumor 1x10rxn\nNormal 1x10rxn\n\n~11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume. RT reaction was stopped by heating at 65 degrees C for 10 minutes. 1ul cDNA is sufficient for one PCR reaction. The 5' end of human clathrin cDNA (a 6kb gene) has been amplified by PCR from all of the cDNAs.\n\nForm:\ncDNA in 1X RT buffer (50 mM Tris Cl, pH 8.3, 75mM KCl, 3mM MgCl2, 10mM DTT). \n\nFeatures:\nReady to use for PCR quantification of gene expression \nOligo dT primer used to ensure the entire 3' end of cDNA is present \nWith some cDNA used as templates, 12kb PCR amplicon was obtained to ensure intact cDNAs \nThe largest selection of cDNAs from different tissues on the market \nPlacenta cDNA is included in every panel as an interpanel control \nDocumentation of tissues' clinical histories available (additional cost) \nEconomical way to get cDNA from multiple tissues \n\nApplications:\nImmediate PCR amplification of known genes for their expression \nQuantification of low copy gene expression in multiple tissues \nIdentification of tissue-specific expression of target genes \nAnalysis of gene expression in rare tissues \nVerification of genetic mutation \nComparison of a specific gene between different tissues \nAnalysis of mRNA alternative splicing\n\nQuality Control:\n1. The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoresed on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detetcted in 10mM Tris-Cl, pH 7.5). The ratio of 28S/18S is > 1.\n\n2. The RNA used for cDNA synthesis is treated by DNase I and is tested as DNA free RNA by PCR.\n\n3. The synthesized cDNA was 5

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