PIAS2 (Protein inhibitor of activated STAT, 2 Androgen, receptor-interacting protein 3, ARIP3, DAB2-interacting protein, DIP, E3 SUMO-protein ligase PIAS2, MGC102682, miz, Miz1, MIZ1, Msx-interacting zinc finger protein, PIAS-NY protein, PIASX, PIASX-AL

PIAS2 (Protein inhibitor of activated STAT2; also DIP, MIZ1 and PIASx-b) is a 45-70kD member of the PIAS family of molecules. In primary cells and tissues it is expressed in T and NK cells, and in testis where it likely regulates spermatogenesis. However, it is widely expressed in cultured cell lines. PIAS2 is known to inhibit STAT4-mediated gene activation, and to interact with inactive ELK-1, promoting its activation via HDAC2 dissociation and desumoylation. Human PIAS2 is 621 amino acids (aa) in length. It contains a SAP domain (aa11-45), a zinc-finger region (aa331-408), a SUMO-binding motif (aa467-472) and an NLS (aa484-492). There are multiple splice variants. PIAS2x-b/ARIP3 shows a 22 aa substitution for aa 551-621, while PIAS-NY shows a 12 aa substitution for aa 1-8, followed by a truncation after Gly401. Two others show a four aa substitution for aa 504-621, and an alternate start site at Met10. Over aa 1-550, human PIAS2 shares 98% aa identity with mouse PIAS2. \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, PBS, 0.02% sodium azide. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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