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Dopamine Beta Hydroxylase (DBH, Dopamine beta Monooxygenase)

Dopamine Beta Hydroxylase (DBH, Dopamine beta Monooxygenase)

Cat no: D9010-13

Supplier: United States Biological
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Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. Belongs to the carnitine/choline acetyltransferase family. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: D9010-13
Reactivities: Human, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
P type: Pab
Purity: Serum
References: Rush R. A. et al (1980) Gut Rev clin Lab Sci 12: 241 ; Verndell I. M. et al (1982) J Histochem Cytochem 30: 682
Additional info: This antiserum has been extensively characterised in several laboratories. It reacts with a single band on western blot of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with the enzyme. Species Crossreactivity: This antibody is known to react with bovine, human and rat DBH.

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