Home  >  Products  >  Dystrophin, NT (Duchenne Muscular Dystrophy Protein, DMD, BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, Muscular Dystrophy Duchenne and Becker Types)
Dystrophin, NT (Duchenne Muscular Dystrophy Protein, DMD, BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, Muscular Dystrophy Duchenne and Becker Types)

Dystrophin, NT (Duchenne Muscular Dystrophy Protein, DMD, BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, Muscular Dystrophy Duchenne and Becker Types)

Cat no: D9910-20A

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
Dystrophin is a rod-like cytoskeletal protein found adja- cent to the muscle membrane. It is one component of a complex system that links actin on the inside of muscle fibers to extracellular matrix proteins that surround the fibers. Mutations in the genes that encode for dys- trophin lead to altered expression of this protein and cause muscular dystrophy. This antibody will help detect dystrophin in normal tissue sections. Applications: Suitable for use in Western Blot and Immunohistochemistry. Not suitable for Immunohistochemistry paraffin sections. Other applications not tested. Recommended Dilution: Western Blot: 1:25-1:50 Immunohistochemistry (Frozen unfixed): Neat-1:20 for 1 hr at RT. Optimal dilutions to be determined by the researcher. Positive Control: Western Blot: Skeletal muscle, doublet of bands at ~400kD. Immunohistochemistry: For reliable interpretation of dystrophin staining results, a spectrin control must be used. Snap frozen normal human striated muscle (staining pattern continuious rim of staining at the periphery of muscle fibers) Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: D9910-20A
Reactivities: Human, Non-Human Primate
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 2.5ml
Form: Supplied as a lyophilized powder from PBS, 1% BSA, 15mM sodium azide. Reconstitute with 2.5ml sterile ddH2O.
P type: Mab
Isotype: IgG2a
Purity: Supernatant
References: 1.Sheriffs I N, Rampling D and Smith V V. Paraffin wax embedded muscle is suitable for the diagnosis of muscular dystrophy. Journal of Clinical Pathology. 54:517-520 (2001).
Additional info: Recognizes Dystrophin. Epitope: binds to regions from aa 308-351. These residues span the junction of exons 9 and 10. The epitope recognized may be part of the hinge region between the amino acid domain and the central rod domain. Species Crossreactivity: human and monkey.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave