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E2F1 (E2F transcription factor 1, Retinoblastoma- binding protein 3)

Cat no: 030153

E2F1 (E2F transcription factor 1, Retinoblastoma- binding protein 3)

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent:independent apoptosis.\n\nApplications:\nWestern Blot: 1:500-1:2000\nImmunohistochemistry: 1:200-1:1000\nFlow Cytometry: 1:200-1:400\nELISA: 1:10000\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

030153

Size

100ul

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer\n<br>

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1.Cancer Res. 2010 Dec 1;70(23):9711-20.\n2.Mol Cancer Ther. 2010 May;9(5):1265-73.\n

Additional Info

Human, Mouse

Alternative Names

RBP3; E2F-1; RBAP1; RBBP3

Read more on Supplier website

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info
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