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E2F1 Transcription Factor (E2F 1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP3, RBP3)

E2F1 Transcription Factor (E2F 1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP3, RBP3)

Cat no: E0010-02F

Supplier: United States Biological
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Clone 16G7 recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry. Positive Control: E2F-1 transfected Soas2 cells and recombinant fusion protein. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E0010-02F
Reactivities: Human
Hosts: Mouse
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 100ug
Form: As reported
P type: Mab
Isotype: IgG1,k
Purity: Mouse ascites
Alternative names: E2F 1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP-3, RBBP3, RBP3
References: 1.Shan, B., Chang, C-Y., Jones, D. and Lee, W-H. (1994) The transcription factor E2F-1 mediates the autoregulation of RB gene expression. Mol. Cell. Biol. 14:299-304. 2.Shan, B., Durfee, T. and Lee, W-H. (1996) Disruption of RB/E2F-1 interaction by single point mutations in E2F-1 enhances S-phase entry and apoptosis. Proc. Natl. Acad. Sci. USA 93:679-684. 3.Shan, B., Farmer, A.A. and Lee, W-H. (1996) The molecular basis of E2F-1/DP-1-induced S-phase entry and apoptosis. Cell Growth and Diff. 7:689-697.
Additional info: This antibody is specific for human E2F-1. Species Crossreactivity: human E2F-1.

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