E2F6 (E2F transcription factor 6, E2F transcription factor 6, isoform 1)

Cat no: E0014-20D

Supplier: United States Biological

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The E2F family of transcription factors includes the E2Fs and DP1. These proteins are critical regulators of genes required for cell cycle progression, and their activity is regulated by association with different protein partners during stages of the cell cycle. E2Fs bind the hypophosphorylated form of pRb, and are maintained in an inactive state. Phosphorylation of Rb causes dissociation, and activation of E2F activity. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunocytochemistry: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	E0014-20D
Reactivities:	Human
Hosts:	Mouse
Applications:	Immunocytochemistry, Immunoprecipitation, Western Blot
Size:	100ug
Form:	Supplied as a liquid in Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
P type:	Mab
Isotype:	IgG1,k
Purity:	Purified by Protein G affinity chromatography.
Additional info:	Recognizes human E2F6.

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E2F6 (E2F transcription factor 6, E2F transcription factor 6, isoform 1)

Cat no: E0014-20D

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