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E2F6 (E2F Transcription Factor 6, E2F Transcription Factor 6, isoform 1)

Cat no: E0014-20E

E2F6 (E2F Transcription Factor 6, E2F Transcription Factor 6, isoform 1)

This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:4000\nWestern Blot: 1-3ug/ml ~33kD band observed in Human Skeletal Muscle lysates (calculated MW of 31.8kD according to NP_937987.2).\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E0014-20E

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1.McLaughlin-Drubin ME, Huh KW, M

Additional Info

Recognizes human E2F6. Species sequence homology: canine, bovine.

Alternative Names

E2F transcription factor 6, isoform 1, MGC111545, E2F-6, E2F transcription factor 6, E2F6

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