EBP50 (ERM (Ezrin-Radixin-Moesin) Binding Phosphoprotein of 50kD)

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50kD] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes. \n\nEBP50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP50 is found exclusively at the apical membranes of epithelial cells. \n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. \n\nRecommended Dilution:\nImmunoprecipitation: 1:50\nWestern Blot: 1:1000\nImmunohistochemistry (paraffin): 1:200\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E1051

Size

100ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG2b

References

1. Saotome, I., et al., (2004) Dev. Cell 6:855-864. 2. Helms, C., et al., (2003) Nat. Genet. 35:349-356. 3. Brdickova, N., et al., (2001) FEBS Lett 507:133-136.

Additional Info

Recognizes human ERM (ezrin/radixin/moesin)-binding phosphoprotein of 50kD (EBP50)/ Na+/H+ exchange regulatory factor (NHERF).