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Ectonucleotide Pyrophosphatase, Phosphodiesterase 5 (E-NPP5, NPP-5, UNQ550/PRO1107)

Ectonucleotide Pyrophosphatase, Phosphodiesterase 5 (E-NPP5, NPP-5, UNQ550/PRO1107)

Cat no: E3224-05

Supplier: United States Biological
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Ectonucleotide pyrophosphatase/phosphodiesterase 5 (ENPP-5) belongs to a group of nucleotide-metabolizing ecto-enzymes, which regulate the availability of extracellular nucleotides. It may also be involved in neuronal cell communication. Applications: Suitable for use in Direct ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human ENPP-5 is ~1ng/well Immunoprecipitation: 25ug/ml will immunprecipitate recombinant human ENPP-5 from conditioned cell culture media Western Blot: 1-2ug/ml. The detection limit for recombinant human ENPP-5 is ~5ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase by 5 to 50 fold. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E3224-05
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes human ENPP-5. In Western Blot shows no cross-recativity with recombinant human ENPP-2 or ENPP-7, or recombinant mouse ENPP-7.

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