EED (embryonic ectoderm development, hEED, HEED, Polycomb protein EED, WAIT1, WAIT-1, WD protein associating with integrin cytoplasmic tails 1)

EED (Embryonic ectoderm development; also WAIT-1) is a 51kD member of the WD repeat ESC family of proteins. It is widely expressed, and constitutes part of both the PRC1 and PRC2 nuclear complexes. PRC1 contains EED, Suz12, RbAp46 and Ezh1 and is found in nonproliferating tissue. PRC2 differs only by the presence of Ezh2 and is found in proliferating tissues. Both complexes methylate histone H3 and act as gene repressors. Human EED is 441aa in length. It contains three phosphorylation sites (Ser34; Thr55; Thr57) followed by seven WD40 domains (aa91-441). There are two alternate start sites, one at Met15, and another that is 94aa upstream of the standard site, begins with a Val and generates a 69kD isoform. A third isoform variant shows an insertion of 25aa after Ser323, while a fourth variant shows a premature truncation after Lys400. Human and mouse EED are identical in amino acid sequence.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nDirect ELISA: 0.5-1ug/ml. The detection limit for recombinant human EED is ~0.2ng/ml.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E2211-50E

Size

100ug

Applications

ELISA, WB

Hosts

Sheep

Reactivities

Hum, Mouse

Form

Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 0.5ml sterile PBS, sterile PBS to prepare a 0.2mg/ml stock solution.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human EED. Species Crossreactivity: mouse.