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EGF (EPIDERMAL GROWTH FACTOR) (Biotin)

Cat no: E3374-15B

EGF (EPIDERMAL GROWTH FACTOR) (Biotin)

EGF (Epidermal Growth Factor), which acts as a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, and is a key factor in cell survival. The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the EGF receptor (EGFR), which results in the triggering of several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.25-1ug/ml\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\nThis antibody may be used in an indirect ELISA or as a detection reagent in a sandwich ELISA with AAR37 as the capture antibody.\n\nRecommended Secondary Antibodies:\nStreptavidin APC\nStreptavidin FITC\nStreptavidin RITC\nStreptavidin RPE, RPE\nStreptavidin HRP\nStreptavidin Alk. Phos.\n\nStorage and Stability: \nLyophilized powder may be stored at 4 degrees C before opening. DO NOT FREEZE! Reconstitute with ddH2O. Aliquot and store at 4C. Stable at 4 degrees C as an undiluted liquid for 6 months. Dilute only prior to immediate use.

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SPECIFICATIONS

Catalog Number

E3374-15B

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Conjugates

Biotin

Form

Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 0.5ml sterile PBS containing 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes rat EGF (Epidermal Growth Factor), a type I transmembrane protein expressed by a variety of cells.

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