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EGF (EPIDERMAL GROWTH FACTOR) (Biotin)

EGF (EPIDERMAL GROWTH FACTOR) (Biotin)

Cat no: E3374-15B

Supplier: United States Biological
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EGF (Epidermal Growth Factor), which acts as a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, and is a key factor in cell survival. The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the EGF receptor (EGFR), which results in the triggering of several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.25-1ug/ml Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. This antibody may be used in an indirect ELISA or as a detection reagent in a sandwich ELISA with AAR37 as the capture antibody. Recommended Secondary Antibodies: Streptavidin APC Streptavidin FITC Streptavidin RITC Streptavidin RPE, RPE Streptavidin HRP Streptavidin Alk. Phos. Storage and Stability: Lyophilized powder may be stored at 4 degrees C before opening. DO NOT FREEZE! Reconstitute with ddH2O. Aliquot and store at 4C. Stable at 4 degrees C as an undiluted liquid for 6 months. Dilute only prior to immediate use.
Catalogue number: E3374-15B
Reactivities: Rat
Hosts: Rabbit
Applications: ELISA, Western Blot
Conjugates: Biotin
Size: 50ug
Form: Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 0.5ml sterile PBS containing 0.1% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes rat EGF (Epidermal Growth Factor), a type I transmembrane protein expressed by a variety of cells.

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