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EGF, ID (Epidermal Growth Factor, egf)

EGF, ID (Epidermal Growth Factor, egf)

Cat no: E3374-15W

Supplier: United States Biological
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EGF (epidermal growth factor) protein is expressed primarily in the ovary and the testis of the zebrafish. Within the ovary, expression of EGF is more abundant in the oocytes. The counterpart to EGF, EGFR (epidermal growth factor receptor), is expressed in nearly all tissues of the zebrafish. Within the ovary, EGFR is expressed in follicle cells with little presence in oocytes. One study suggests that EGF is a factor through which oocytes thus regulate follicle cells. In humans, EGF stimulates growth in epidermal and epithelial tissue, under both in vivo and in vitro conditions. Defective EGF in humans is the cause of hypomagnesemia type 4. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:5000-1:20,000 Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher. Control Peptide: E3374-15Z: EGF, ID, Control Peptide (Epidermal Growth Factor, egf) Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E3374-15W
Reactivities: Zebrafish/Fish
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry
Size: 250ul
Form: Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Wang, Y and Ge, W (2004) Biol. Reprod. 71(3): 749-760. 2. Groenestege W.M.T (2007) J. Clin. Invest. 117: 2260-2267.
Additional info: Recognizes zebrafish EGF.

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