EHD2 (EH Domain Containing 2, PAST2)

EHD2 is homologous to human EHD1. The deduced protein identities between EHD1 and EHD2, between EHD1 and EHD3, and between EHD1 and EHD4, are 71%, 86%, and 76%, respectively. All contain multiple conserved regions that include a nucleotide binding consensus site at the N terminus, a bipartite nuclear localization signal, and an eps15 homology (EH) protein binding domain with an EF hand motif at the C terminus.\nNorthern blot analysis detected a EHD2 transcript that is highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle, as well as different transcript that is expressed at low levels in heart and skeletal muscle.\n\nCellular Localization: Nuclear\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E2215-80

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat, ZF/Fish

Form

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Guilherme A et al., (2004) J Biol Chem 279:40062-75.

Additional Info

This antibody is expected to recognize EHD1 protein as well as EHD2. Species Crossreactivity: Reacts with Human. Predicted to react with Mouse (99% identity with immunogen), Rat (99% identity with immunogen), bovine (100% identity with immunogen) and Zebrafish (99% identity with immunogen) due to sequence homology.