EHD2 (EH Domain Containing 2, PAST2)

This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:32,000\nWestern Blot: 0.003-0.1ug/ml ~ 60kD band observed in Human Heart lysates (calculated MW of 61.2kD according to NP_055416.2). In transfected HEK293 transiently expressing EHD2 a band of approx. 62kD is observed. This band is not observed in the non-transfected HEK293.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2215-80C

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1. Doherty KR, Demonbreun AR, Wallace GQ, Cave A, Posey AD, Heretis K, Pytel P, McNally EM.\nThe endocytic recycling protein EHD2 interacts with myoferlin to regulate myoblast fusion.\nJ Biol Chem. 2008 Jul 18;283(29):20252-60

Additional Info

Recognizes human EHD2. Species sequence homology: mouse, rat, canine, bovine.\nThis antibody is expected to recognise EHD1 protein as well as EHD2.

Alternative Names

FLJ96617, EH domain containing 2, EH-domain containing 2, PAST2, EHD2