EIF2AK3 (PEK, PERK, HsPEK, Pancreatic eIF2-alpha kinase, PRKR-like endoplasmic reticulum kinase, Eukaryotic translation initiation factor 2-alpha kinase 3)

EIF2AK3 (Eukaryotic Translation Initiation Factor 2-Alpha Kinase 3), also called PEK, is an enzyme that in humans is encoded by the EIF2AK3 gene. By fluorescence in situ hybridization and radiation hybrid analysis, Hayes et al. (1999) mapped the EIF2AK3 gene to chromosome 2p12.Harding et al. (2000) reported that a targeted mutation of the mouse Eif2ak3 gene, which they called Perk, abolished the phosphorylation of eIF2-alpha in response to accumulation of malfolded proteins in the ER, resulting in abnormally elevated protein synthesis and higher levels of ER stress. Using a library of endoribonuclease-prepared short interfering RNAs (esiRNAs), Kittler et al. (2004) identified 37 genes required for cell division, one of which was EIF2AK3. \n\nUniProt Number:\nQ9NZJ5\n\nGene ID:\nEIF2AK3\n\nApplications:\nSuitable for use in Western Blot and Immunocytochemistry.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144624

Size

100ug

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Harding, H. P., Zhang, Y., Bertolotti, A., Zeng, H., Ron, D. Perk is essential for translational regulation and cell survival during the unfolded protein response. Molec. Cell 5: 897-904, 2000. \n2. Hayes, S. E., Conner, L. J., Stramm, L. E., Shi, Y. Assignment of pancreatic eIF-2a kinase (EIF2AK3) to human chromosome band 2p12 by radiation hybrid mapping and in situ hybridization. Cytogenet. Cell Genet. 86: 327-328, 1999. \n3. Kittler, R., Putz, G., Pelletier, L., Poser, I., Heninger, A.-K., Drechsel, D., Fischer, S., Konstantinova, I., Habermann, B., Grabner, H., Yaspo, M.-L., Himmelbauer, H., Korn, B., Neugebauer, K., Pisabarro, M. T., Buchholz, F. An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature 432: 1036-1040, 2004.\n

Additional Info

Recognizes human h.EIF2AK3. No crossreactivity with other proteins.