eIF3B (Eukaryotic Translation Initiation Factor 3 Subunit B, eIF3b, Eukaryotic Translation Initiation Factor 3 Subunit 9, Prt1 Homolog, hPrt1, eIF-3-eta, eIF3 p110, eIF3 p116, EIF3S9)

eIF3B is an acidic protein belonging to the EIF-3 subunit B family with an RRM (RNA recognition motif) domain. An important protein involved in protein translation:synthesis, eIF3B is known to bind to the 40S ribosomal subunit and promotes the binding of methionyl-tRNAi and mRNA. eIF3B is a component of the EIF3 multiprotein complex, composed of at least 13 different subunits. It is also known to interact with eIF3A and directly with the p170 subunit of EIF3, the region required for this association is located within the RRM. Ubiquitous expression of eIF3B is seen in most of the tissues.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.05-0.25ug/ml\nImmunohistochemistry (formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E2238-20C

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Asano, K. et al. J. Biol. Chem. 272:1101-1109 (1997). 2. Chaudhuri, J. et al. J. Biol. Chem. 272:30975-30983 (1997). 3. Methot, N. et al. J. Biol. Chem. 272:1110-1116 (1997).

Additional Info

Recognizes human eIF3B. Species Crossreactivity: mouse, rhesus monkey. Species sequence homology: bovine, canine, equine, opossum, rat.