EIF3M (PCID1, Eukaryotic Translation Initiation Factor 3 Subunit M, eIF3m, Fetal Lung Protein B5, hFL-B5, PCI Domain-containing Protein 1, HFLB5, GA17, PNAS-125)

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2/GTP/methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2).\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:32,000\nWestern Blot: 1-3ug/ml, observed in lysates of human lung carcinoma cell line A549 on ~38-40kD bands\nImmunohistochemistry (Paraffin): 5-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E8949-37T1

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat, Bov, Can

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Perez-Romero P, Fuller AO. The C terminus of the B5 receptor for herpes simplex virus contains a functional region important for infection. J Virol. 2005 Jun;79(12):7431-7.

Additional Info

Recognizes human EIF3M. Species sequence homology: Bovine, canine, mouse and rat.