Elafin/PI3 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human Elafin/PI3 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nElafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase (SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.\n\nAssay Range:\n31.2pg/ml-2000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP19957 \n\nEntrez Gene ID:\n5266\n\nKit Components:\n96-well plate pre-coated with anti-Elafin/PI3, Human\nLyophilized recombinant Elafin/PI3, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143816

Size

96Tests

References

1. Ghosh, M., Shen, Z., Fahey, J. V., Cu-Uvin, S., Mayer, K., Wira, C. R. Trappin-2/Eliafin: a novel innate anti-human immunodeficiency virus-1 molecule of the human female reproductive tract. Immunology 129: 207-219, 2009. \n2. King, A. E., Critchley, H. O. D., Sallenave, J.-M., Kelly, R. W. Eliafin in human endometrium: an antiprotease and antimicrobial molecule expressed during menstruation. J. Clin. Endocr. Metab. 88: 4426-4431, 2003.\n