Elk-1, aa307-428, Recombinant, Human

Elk-1 is a transcription factor that is activated by mitogen-activated protein kinases (MAPKs) and whose main function is the regulation of growth-related proteins in response to extracellular stimuli. Because of the importance of Elk-1 to the regulation of successful cell growth, accurate monitoring of Elk-1 in cells, tissues and animals is crucial for many biomedical research and drug development projects.\n\nDescription: \nRecombinant Human ELK-1 produced in E.Coli is a non-glycosylated, polypeptide chain containing 122 amino acids (307-428) and having a molecular mass of 60kD. This protein is conjugated with N-terminal His-tag and C-terminal myc tag (EQKLISEEDL). Recombinant Elk-1 is purified by proprietary chromatographic techniques.\n\nBiological Activity: \nRecombinant human Elk-1 is phosphorylatable in vitro, using either recombinant active ERK1/2, or with immunoprecipitated ERK1/2 from stimulated cells. This phosphorylation can be monitored by Western blot analysis using BioSource's phosphorylation site specific antibody directed to Elk-1 [pS383] in conjunction with chemiluminescence detection methods. Please note: Kinase activity may vary depending on the substrate and reaction conditions.\n\nStorage: \nStore at 4 degrees C if entire vial will be used within 1-2 weeks. Store, frozen at -20 degrees C for longer periods of time. Please avoid freeze-thaw cycles.

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