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Embryonic Lysate, 48hpf, Zebrafish

Cat no: E2256-20A

Embryonic Lysate, 48hpf, Zebrafish

Zebrafish embryos were collected 48 hours post fertilization (48 hpf), homogenized in modified RIPA buffer and cleared by centrifugation. Prior to lysis, embryos were treated to remove majority of the embryonic yolk. Total protein concentration of the embryo lysate was determined using BCA protein assay (BSA was used as a standard). Lysate was mixed with 2X Laemmli sample buffer containing reducing reagent and heated for 5 minutes at 90 degrees C. E2256-20A is supplied at 2mg/ml total protein concentration in 1x laemmli sample buffer.\n\nApplications:\nSuitable for use in Western Blot and SDS-PAGE electrophoresis. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 15-20ug of total protein per lane\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2256-20A

Size

200ug

Applications

WB

Form

Supplied as a liquid in RIPA buffer and 1x Laemmli sample buffer. (RIPA buffer: 150mM sodium chloride, 50mM Tris-Cl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 1mM EDTA, 1mM PMSF, 5ug/ml Pepstatin A, 2ug/ml Leupeptin, 1mM sodium orthovanadate, 1mM sodium fluoride).

Purity

Lysate

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