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Emerin (Emery-Dreifuss Muscular Dystrophy, EDMD, EMD, LEM Domain Containing 5, LEMD5, STA)

Emerin (Emery-Dreifuss Muscular Dystrophy, EDMD, EMD, LEM Domain Containing 5, LEMD5, STA)

Cat no: E2257-02B

Supplier: United States Biological
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Emerin is a broadly expressed integral protein of the nuclear inner membrane (1). It contains a LEM domain and binds to several nuclear proteins, such as BAF (barrier-to-autointegration factor) and A- and B-type lamins, which are important in nuclear functions (2-5). Emerin may regulate gene expression through binding to other transcriptional regulators (6,7). Emerin binds to B-catenin and inhibits its nuclear accumulation (8). Recent studies demonstrate that Emerin is required for HIV-1 infectivity (9). Mutations in Emerin are a major cause of Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive skeletal muscle weakening (10). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E2257-02B
Reactivities: Human
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Purified
References: (1) Nagano, A. et al. (1996) Nat. Genet. 12, 254-259. (2) Manilal, S. et al. (1998) Biochem. Biophys. Res. Commun. 249, 643-647. (3) Clements, L. et al. (2000) Biochem. Biophys. Res. Commun. 267, 709-714. (4) Lee, K.K. et al. (2001) J. Cell Sci. 114, 4567-4573. (5) Bengtsson, L. and Wilson, K.L. (2006) Mol. Biol. Cell 17, 1154-1163. (6) Holaska, J.M. et al. (2003) J. Biol. Chem. 278, 6969-6975. (7) Haraguchi, T. et al. (2004) Eur. J. Biochem. 271, 1035-1045. (8) Markiewicz, E. et al. (2006) EMBO J. 25, 3275-3285. (9) Jacque, J.M. and Stevenson, M. (2006) Nature 441, 641-645. (10) Holaska, J.M. and Wilson, K.L. (2006) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 288, 676-680.
Additional info: Recognizes endogenous level of total Emerin.

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