Emerin (Emery-Dreifuss Muscular Dystrophy, EDMD, EMD, LEM Domain Containing 5, LEMD5, STA)

Emerin is a broadly expressed integral protein of the nuclear inner membrane (1). It contains a LEM domain and binds to several nuclear proteins, such as BAF (barrier-to-autointegration factor) and A- and B-type lamins, which are important in nuclear functions (2-5). Emerin may regulate gene expression through binding to other transcriptional regulators (6,7). Emerin binds to B-catenin and inhibits its nuclear accumulation (8). Recent studies demonstrate that Emerin is required for HIV-1 infectivity (9). Mutations in Emerin are a major cause of Emery-Dreifuss\nmuscular dystrophy (EDMD), a disorder characterized by progressive skeletal muscle weakening (10).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS,\n0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2257-02B

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

(1) Nagano, A. et al. (1996) Nat. Genet. 12, 254-259. (2) Manilal, S. et al. (1998) Biochem. Biophys. Res. Commun. 249, 643-647. (3) Clements, L. et al. (2000) Biochem. Biophys. Res. Commun. 267, 709-714. (4) Lee, K.K. et al. (2001) J. Cell Sci. 114, 4567-4573. (5) Bengtsson, L. and Wilson, K.L. (2006) Mol. Biol. Cell 17, 1154-1163. (6) Holaska, J.M. et al. (2003) J. Biol. Chem. 278, 6969-6975.\n(7) Haraguchi, T. et al. (2004) Eur. J. Biochem. 271, 1035-1045. (8) Markiewicz, E. et al. (2006) EMBO J. 25, 3275-3285. (9) Jacque, J.M. and Stevenson, M. (2006) Nature 441, 641-645. (10) Holaska, J.M. and Wilson, K.L. (2006) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 288, 676-680.

Additional Info

Recognizes endogenous level of total Emerin.