Encephalitis, Tick-borne, NS3, Recombinant

E.coli derived recombinant. The protein contains the Tick-borne Encephalitis Virus Nonstructural protein NS3 (Flavivirin protease NS3 catalytic subunit) epitopes.\n\nSpecificity: \nImmunoreactive with sera of encephalitis virus infected individuals.\n\nApplication: \nAntigen in ELISA and Western blots, excellent antigen for detection of Tick-borne encephalitis virus with minimal specificity problems.\n\nStorage: \nProtein is shipped at ambient temperature. Upon arrival, Store at -20 degrees C.\n\nStability: \nFive years frozen. One month in solution at room temperature.

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SPECIFICATIONS

Catalog Number

E2280-74

Size

100ug

Form

Supplied as a liquid in 20mM MES, pH 6.5, 8M urea, 200mM sodium chloride and 0.05% Tween-20.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Japanese encephalitis virus NS2B-NS3 protease binding to phage-displayed human brain proteins with the domain of trypsin inhibitor and basic region leucine zipper. Virus Res 2006 Mar;116(1-2):106-13. 2. Suramin inhibits the in vitro expression of encephalitis B virus proteins NS3 and E. J Huazhong Univ Sci Technolog Med Sci 2003;23(4):375-9. 3. Association of Japanese encephalitis virus NS3 protein with microtubules and tumour susceptibility gene 101 (TSG101) protein. J Gen Virol 2003 Oct;84(Pt 10):2795-805. 4. Mutations in the NS3 gene and 3'-NCR of Japanese encephalitis virus isolated from an unconventional ecosystem and implications for natural attenuation of the virus. Virology 2001 Oct 10;289(1):129-36. 5. Role of the DExH motif of the Japanese encephalitis virus and hepatitis C virus NS3 proteins in the ATPase and RNA helicase activities. Virology 2000 Aug 1;273(2):316-24. 6. Tick-borne encephalitis virus NS3 gene expression does not protect mice from homologous viral challenge. Viral Immunol 1999;12(4):277-80.