Home  >  Products  >  EOMES (Eomesodermin Homolog, T-box Brain Protein 2, T-brain-2, TBR-2, TBR2)
EOMES (Eomesodermin Homolog, T-box Brain Protein 2, T-brain-2, TBR-2, TBR2)

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, T-brain-2, TBR-2, TBR2)

Cat no: E3320-01C

Supplier: United States Biological
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EOMES is a 72kD member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-g and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunocytochemistry: 8-25ug/ml Flow Cytometry: 2.5ug/106 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E3320-01C
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunocytochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
P type: Mab
Isotype: IgG2B
Purity: Purified by Protein A/G affinity chromatography.
Additional info: Recognizes human EOMES. No cross-reactivity with recombinant human Brachyury, rhEOMES (aa1-115), recombinant mouse EOMES (aa1-126), rhTBX2, 3, 5, 6, 18, or 20.

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