Ephrin A3 (EPH-related receptor tyrosine kinase ligand 3)

Ephrins are a family of proteins that bind to the tyrosine kinase Eph receptor. Their functions are still under investigation, but they are suspected to play an important role in morphogenesis. The ephrins can be grouped into two classes: those that are tethered to the membrane by a GPI linkage, called ephrin-A proteins (which bind to EphA receptors), and those with a membrane-spanning region, called ephrin-B proteins (which bind to EphB receptors). ephrins are expressed in the embryo in various tissues in the forming mesoderm, endoderm and nervous system in all of the vertebrate species in which they have been identified. ephrin-A3/EphA4 signaling has been shown to control hippocampal dendritic spine morphology, while ephrin-A3/EphA5 signaling plays an important role in entorhino-hippocampal axon targeting.\n\nApplications:\nSuitable for use in Western Blot, ELISA and Immunofluorescence. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 1-3ug/ml. Identifies a positive band at ~60kD and an additional band at ~30kD that is suspected to be the result of a breakdown product.\nImmunofluorescence: 10-15ug/ml. Stains full-length ephrin-A3 transfected-HEK293 cells and frozen mouse hippocampus tissue sections.\nELISA: 0.1-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E3365-04

Size

100ug

Applications

ELISA, IF, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by epitope affinity chromatography.

Isotype

IgG

References

1. Murai KK, et al. Nat Neurosci 6: 153-160, 2003. 2. Holder N and Klein R. Development 126(10): 2033-2044, 1999. 3. Stein E, et al. J Neurosci 19(20): 8885-8893, 1999.

Additional Info

Recognizes mouse ephrin-A3 recombinant protein. Reactivity in Western Blot has been confirmed with mouse ephrin-A3 recombinant protein. Species sequence homology: human.