Epidermal Growth Factor Receptor (erbB, EGFR, EC=2.7.10.1, ERBB, ERBB1, MENA)

EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), transforming Growth Factor-a (TGFa), Heparin-binding EGF (HB-EGF), emphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.\n\nCellular Localization: Cell Membrane and Secreted\n\nPositive Control: A431 cells.\n\nApplications: \nSuitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3375-01L

Size

500ul

Applications

FC, IP

Hosts

Mouse

Reactivities

Hum, Can

Form

10mM PBS, pH7.4, 0.2%BSA

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Wikstrand CJ et al. (1995). Cancer Res 55:3140-8. 2. Hartigh JC et al. (1993) Biochim Biophys Acta 1148:249-56. 3. Kawamoto T, et al., (1983) Proc Natl Acad Sci USA 80:1337-41. 4. Sato JD et al. (1983) Mol Biol Med 1:511-29.

Additional Info

Species Crossreactivity: Crossreacts with Human and canine. Does not crossreact with Rat or Mouse.

Alternative Names

EC=2.7.10.1, ERBB, ERBB1, MENA