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Epiregulin, Recombinant, Human (EREG)

Cat no: E3376-75

Epiregulin, Recombinant, Human (EREG)

Epiregulin is an EGF related growth factor that binds specifically to EGFR (ErbB1) and ErbB4, but not ErbB2 or ErbB3. It is expressed mainly in the placenta and peripheral blood leukocytes and in certain carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and vascular smooth muscle cells. It also inhibits the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0kD transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0kD mature secreted sequence. Recombinant human Epiregulin is a 6.0kD monomeric protein, containing 50 amino residues, which corresponds to the mature secreted Epiregulin sequence.\n\nAA Sequence:\nMVAQVSITKC SSDMNGLYCLH GQCIYLVDMS QNYCRCEVGY TGVRCEHFFL\n\nBiological Activity:\nThe ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is 0.3ug/ml in the presence of 15ng/ml of human IGF-II. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O and carrier protein. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nCountry of Origin:\nUSA

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SPECIFICATIONS

Catalog Number

E3376-75

Size

5ug

Form

Supplied as a lyophilized powder with no additives. \nReconstitute with sterile ddH20, carrier protein to 0.1-1mg/ml.

Purity

(same/more than)98% by SDS-PAGE and HPLC analyses.

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