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Epithelial Membrane Antigen (EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin, MUC-1)

Cat no: E3414-15D

Epithelial Membrane Antigen (EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin, MUC-1)

Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP \n\nRecommended Positive Control Tissue: Normal Epithelia\n\nApplications:\nImmunohistochemistry: (frozen)- 1:10-1:40, (paraffin)- 1:10-1:40\nOptimal working dilutions to be determined by researcher.\n\nImmunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

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SPECIFICATIONS

Catalog Number

E3414-15D

Size

1ml

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Tissue Culture Supernatant. Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.

P Type

Mab

Purity

Tissue culture supernatant.

Isotype

IgG2a

References

1. Gusev, Y., et al., (2001) American Journal of Pathology 159(1):63-69.

Additional Info

Recognizes epithelial membrane antigen (EMA), a heavily glycosylated protein of 265-400kD.This antibody stains normal and neoplastic epithelium including adenocarcinomas from a range of sites, squamous and transitional cell carcinomas and mesotheliomas. Strong staining of glandular epithelium is seen, but staining of squamous epithelium may be rather patchy. Positive staining with this antibody demonstrates epithelial origin of tumors.

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