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ERMAP (Erythrocyte Membrane-associated Protein, Scianna Blood Group Antigen, RD, SC) (APC)

Cat no: 145459

ERMAP (Erythrocyte Membrane-associated Protein, Scianna Blood Group Antigen, RD, SC) (APC)

ERMAP (erythrocyte membrane-associated protein; also known as Scianna blood group antigen) is a 6066kD member of the BTN/MOG family, Ig superfamily of proteins. It is expressed on erythrocytes and erythrocyte precursors and likely serves as a cell adhesion molecule. Mature human ERMAP is a 446 amino acid (aa) type I transmembrane glycoprotein. It possesses a 126 aaextracellular domain (ECD; aa30-155) that contains one V-type Ig-like domain (aa30-140). Single aachanges at Gly35, Glu47, Gly57, Pro60, and Arg81 generate distinct antigens of the Scianna blood group. There is one alternate start site at Met91 and a splice variant that shows an 11aa substitution for aa103-475. Over aa30-155, human ERMAP is 56% aa identical to mouse ERMAP. In contrast to the extracellular domain of human ERMAP, the ECD of mouse ERMAP contains one extra Ig-like domain.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 10ul, 1x10e6 cells \nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nMouse myeloma cells with B cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at 12 months at 4 degrees C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyaninconjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

145459

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).

P Type

Mab

Purity

Purified by Protein A or G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human ERMAP.

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