Erythrina cristagalli Lectin (ECA, ECL)

Erythrina cristagalli lectin is a 54,000D glycoprotein consisting of two different subunits of approximately 28,000 and 26,000D. It has a specificity toward galactose residues and appears to have the highest binding activity toward galactosyl (b -1,4) N-acetylglucosamine.\nThis lectin has been reported to be useful for the isolation of human natural killer (NK) cells using a negative selection panning technique (protocol available upon request). Human NK cells appear to lack accessible surface carbohydrate structures required for binding ECL and, unlike other mononuclear cells, do not adhere to ECL-coated culture dishes. Since this procedure involves a negative selection panning technique, a high recovery of viable NK cells can be obtained. The adherent cells can also be recovered by incubation in galactose or lactose.\nThe carbohydrate structure to which ECL binds is frequently found in membrane and serum glycoproteins of mammalian origin. Sialic acid substitution on this structure appears to prevent the lectin from binding. This specificity offers an opportunity to utilize agarose bound ECL to isolate or fractionate mammalian glycoproteins. ECL has also been reported to be mitogenic to human peripheral T lymphocytes but is not mitogenic toward human peripheral B lymphocytes, mouse splenocytes or mouse thymocytes.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nInhibiting/Eluting Sugar: \n200mM lactose\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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