Escherichia coli, O157 (E. coli) (FITC)

E. coli O157:H7 is an enterohemorrhagic strain of the bacterium E. coli and a cause of foodborne illness. It is a gram-negative rod-shaped bacterium. The letter "O" (not a zero) in the name refers to the somatic antigen number, whereas the "H" refers to the flagella antigen. E. coli O157:H7 is markedly different from other pathogenic E. coli. In particular, the O157:H7 serotype is negative for invasiveness (sereny test), elaborates no colonization factors (CFA/I or CFA/II), doesn't produce heat stable or heat labile toxins and is non-hemolytic. In addition, E. coli O157:H7 is usually sorbitol negative whereas 93% of all E. coli ferment sorbitol. E. coli O157:H7 also lacks the ability to hydrolyze 4-methylumbelliferyl-beta-D-glucuronide (MUG) and does not grow at 45 degrees C in the presence of 0.15% bile salts. Because of the latter characteristic this serotype cannot be isolated by using standard fecal coliform methods that include incubation at 45 degrees C.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence: 1:5-1:20 Acetone fixation of the antigen source is recommended prior to staining.\nELISA: 1:5-1:20\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E3500-39A

Size

1ml

Applications

ELISA, IF, IP

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Labeled with FITC.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG3

References

General References:\n1. \"Escherichia coli O157:H7\". CDC Division of Bacterial and Mycotic Diseases. Retrieved on 2007-01-25.\n2. Vogt RL, Dippold L (2005). \"Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June-July 2002\". Public Health Rep 120 (2): 174

Additional Info

Recognizes E. coli serotype O157. Immunoprecipitates LPS of O157:H7.