Estrogen Receptor-a, phosphorylated (Ser118) (ER, ER-alpha, Estradiol Receptor, Nuclear Receptor Subfamily 3 Group A Member 1, ESR1, ESR, NR3A1)

Estrogen receptor-a is a ligand activated, transactivating protein. The wild type estrogen receptor-a coded by a 6.6kb mRNA, comprised of five functional domains derived from eight exons. The three domains of the protein which show the greatest degree of sequence homology are the A domain which, in concert with the B domain, contains the transactivation function (AF-1), the C domain which contains two zinc-fingers and is responsible for DNA binding and the E domain which is responsible for ligand binding as well as the ligand inducible transactivation function (AF-2). Amplification of the protein is a common mechanism in proliferative breast disease. Estrogen receptor-a IVS1-397T/C polymorphism is associated with risk of myocardial infarction and other cardiovascular disease in both men and women. Human estrogen receptor-a gene is mapped to chromosome 6q25.1.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (paraffin): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMCF7 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3565-10S

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Pink, JJ. et al. Nucleic. Acids. Res. 24:962-969 (1996). 2. Frederik, H. et al. Nature Genetics. 39:655-660 (2007). 3. Alisa, D. et al. Kjaergaard Circulation. 115:861-871 (2007).

Additional Info

Recognizes human Estrogen Receptor-a, phosphorylated at Ser118. Species Crossreactivity: mouse.