Estrogen Receptor alpha, Phosphorylated (Ser305) (ER, ER alpha, Era, ESR, ESR, ESR1, ESRA, Estradiol Receptor, Estrogen Receptor, Estrogen Receptor 1)

Estrogen Receptor a is a nuclear hormone receptor. This steroid hormone receptor is involved in the regulation of eukaryotic gene expression and affects cellular proliferation and differentiation in target tissues. ERA is a ligand-activated transcription factor and upon being bound by a ligand such as estrogen, a conformational change occurs that allows dimerization and binding to an estrogen response element. When bound to DNA, Estrogen Receptor A can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Two ERs are known, ERA, classically known as ER, and ERB. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERB is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERA, the molecular mechanisms regulating the transcriptional activity of ERB appears to be distinct from those regulating ERA.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nImmunohistochemistry: 1:200\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nPKA phosphorylated and unphosphorylated recombinant ERA protein\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029467

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Figtree, G.A., et al. (2009). Recent Pat DNA Gene Seq. 3(3):164-171. 2. Rayala, S.K., et al. (2006). J Biol Chem. 281(28):19092-19099. 3. Rayala, S.K., et al. (2005). EMBO Rep. 6(6):538-544.

Additional Info

Recognizes human Estrogen Receptor alpha phosphorylated at Ser305.