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Ethenoadenosine

Ethenoadenosine

Cat no: E8490-01A

Supplier: United States Biological
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Ethenobases are adduct products of vinyl chloride, an occupational carcinogen. The sensitivity and specificity of this ethenoadenosine antibody have been determined by enzyme-linked immunosorbent assay. It reacts with both the ribose and deoxyribose form of the adduct. In addition, previous lots of this antibody have been shown to detect etheno-ADP-ribosylation of cell surface proteins by flow cytometry. Cellular Localization: Nuclear Applications: Suitable for use in Immunofluorescence, ELISA and Immunocytochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E8490-01A
Hosts: Mouse
Applications: ELISA, Immunocytochemistry, Immunofluorescence
Size: 100ul
Form: As reported
P type: Mab
Isotype: IgG2,l
Purity: Purified by Protein G affinity chromatography.
References: 1. Krebs, C. et al, (2003) Anal Biochem. 314(1):108-115. 2. Young, TL. et al, (1988) Carcinogenesis. 9(4):589-592. 3. Grahnert, A. et al, ( 2002) Biochem. J. 362:717-723. 4. Ohlrogge, W. et al, (2002) Mol. and Cell Biol. 22(21):7535-7542. 5. Liao, S. D., and Puro, D. G. (2006) 47:5032-5038.
Additional info: This antibody is specific for ethenoadenosine and ethenod-adenosine. Species Crossreactivity: all

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