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Ethinylestradiol(6)

Cat no: E8460-01A


Supplier: United States Biological
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Ethinylestradiol is a synthetic estrogen with high oral hormonal potency. (1) It is rapidly reabsorbed from the gastrointestinal tract and the presence of an ethinyl at the 17-position reduces hepatic first-passage metabolism. (1) It is excreted in urine and some is found in faeces. Ethinylestradiol is illegally used in animal husbandry as an anabolic (1) and is excreted as the parent molecule in faeces, and as glucuronide or sulphate tracer in urine. (2) Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 2.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E8460-01A
Hosts: Sheep
Applications: ELISA
Size: 100ul
Form: Supplied as a liquid in 20mM phosphate, 150mM sodium chloride, pH 7.2, 0.09% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified
References: 1. Standardization of Hormone and Veterinary Drug Residue Analysis in Animal Products, Molecule Database. (http://cemu10.fmv.ulg.ac.be/OSTC/default.html). 2. Huang CH, Sedlak DL. Analysis of estrogenic hormones in municipal wastewater effluent and surface water using enzyme-linked immunosorbent assay and gas chromatography/tandem mass spectrometry. Environ Toxicol Chem. 2001, 20(1): 133-9.
Additional info: Recognizes ethinylestradiol.

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