Eukaryotic Translation Initiation Factor 2, alpha (eIF2a)

EIF-2alpha, also known as eukaryotic translation initiation factor 2alpha, promotes the binding of the initiator tRNA to the 40S ribosomal subunits and controls translational rates via phosphorylation/dephosphorylation mechanisms. The double-stranded RNA (dsRNA)-activated protein kinase (PKR) provides an important control in the regulation of protein synthesis initiation through the phosphorylation of eIF-2alpha. Phosphorylated eIF-2 alpha inhibits cell growth and induces apoptosis (1). Phosphorylation of eIF-2alpha occurs under a variety of conditions including viral infection, apoptosis, nutrient deprivation, heme-deprivation, and certain stresses (2). EIF-2 exists as a heterotrimeric protein composed of 3 nonidentical subunits, alpha (36kD), beta (38kD), and gamma (52 kD) (3).\n \nApplications:\nSuitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot (ECL): 1ug/ml \nOptimal dilutions to be determined by researcher.\n \nPositive Controls:\nHeLa Cell Lysate: Use 20ug per lane.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E8949-01

Size

50ug

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, GP, NHP, Prc, Rabbit, Sheep

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kumar, K.U., et al. (1999) Mol. Cell Biol. 19:1116-1125. 2. DeGracia, D.J., et al., (1997) J. Cereb. Blood Flow Metab. 17:1291-1302. 3. Lobo, M.V.,et al(1997) Histochem. J. 29:453-68. 4. Brewer, J.W. and Diehl, J.A. (2000). PNAS. 97(23):12625-12630. 5. Kedersha, N., et al. (2002) Mol Biol Cell 13(1):195-210.

Additional Info

Recognizes a ~36kD protein, corresponding to the apparent molecular mass of rat eIF-2alpha. Species Crossreactivity: human, mouse, bovine, canine, guinea pig, hamster, monkey, porcine, rabbit and sheep.