Home  >  Products  >  Eukaryotic Translation Initiation Factor 2, alpha (Eukaryotic Tanslation Initiation Factor 2 Subunit alpha, eIF-2alpha, eIF-2-alpha, eIF2a, eIF-2A, EIF2, EIF-2, Eukaryotic Translation Initiation Factor 2 Subunit 1, EIF2S1)
Eukaryotic Translation Initiation Factor 2, alpha (Eukaryotic Tanslation Initiation Factor 2 Subunit alpha, eIF-2alpha, eIF-2-alpha, eIF2a, eIF-2A, EIF2, EIF-2, Eukaryotic Translation Initiation Factor 2 Subunit 1, EIF2S1)

Eukaryotic Translation Initiation Factor 2, alpha (Eukaryotic Tanslation Initiation Factor 2 Subunit alpha, eIF-2alpha, eIF-2-alpha, eIF2a, eIF-2A, EIF2, EIF-2, Eukaryotic Translation Initiation Factor 2 Subunit 1, EIF2S1)

Cat no: E8949-09E

Supplier: United States Biological
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Phosphorylation of the eukaryotic initiation factor 2 (eIF2) a subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK),aa deprivation (GCN2) or heme deficiency (HRI) can phosphorylate the a subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-y and TNF-a induces potent phosphorylation of eIF2a at Ser51 (5,6). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunohistochemistry (Pafarrin):1:800 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E8949-09E
Reactivities: Human, Mouse, Rat, Non-Human Primate
Hosts: Rabbit
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Purified
Alternative names: Eukaryotic Translation Initiation Factor 2, Subunit 1 alpha, 35kDa
References: (1) Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31,25-29. (2) De Haro, C. et al. (1996) FASEB J. 10, 1378-1387. (3) Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233. (4) Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128. (5) Cheshire, J.L. et al. (1999) J. Biol. Chem. 274,4801-4806. (6) Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.
Additional info: Recognizes endogenous levels of total eIF2a. Species Crossreactivity: monkey, mouse, rat.

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