Eukaryotic Translation Initiation Factor 2, alpha, phosphorylated (Ser51) (eIF2a)

Eukaryotic initiation factor 2 (eIF2) plays a central role in initiating translation, and provides for a rate-limiting step in protein synthesis. Phosphorylation of a-subunit of eIF2 effectively prevents formation of the eIF2/GTP/Met-tRNAi complex and inhibits global protein synthesis. Three distinct protein kinases regulate protein synthesis in eukaryotic cells by phosphorylating the a-subunit of eIF2 at Serine51. Phosphorylation occurs under a wide variety of different stimuli, including heat shock, serum deprivation, glucose starvation, amino acid starvation, exposure to heavy metal ions, and viral infection.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry, Flow Cytometry and Dot Blot. NOt recommended for use in Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:500. Detects a band at ~36kD\nImmunohistochemistry: Frozen and paraffin sections\nImmunocytochemistry (IF): 1:100-1:250\nFlow Cytometry: 1:100\nDot Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nPC12 cell lysate, human liver carcinoma\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E8949-01B

Size

100ul

Applications

FC, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Dro/Arth

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% BSA, 0.01% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Ernst, H., et al. Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2. Characterization of the protein and its messenger RNA. J Biol Chem. 262: 1206

Additional Info

Recognizes human eIF-2a phosphorylated at serine 51. Species Crossreactivity: mouse and rat, Drosophila melanogaster, plants, African Green Monkey, Neurospora crassa