Eukaryotic Translation Initiation Factor 2, alpha, phosphorylated (Ser51) (eIF2a)

Phosphorylation of the eukaryotic initiation factor 2 (eIF2) alpha subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) or heme deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-gamma and TNF-alpha induces potent phosphorylation of eIF2alpha at Ser51 (5,6)\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1:1000 \nImmunoprecipitation: 1:100 \nImmunohistochemistry (Paraffin): 1:50 IHC protocol: Unmasking buffer/Antibody diluent \nCitrate/TBST-5%NGS \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E8949-03

Size

100ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Dro/Arth, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.

Additional Info

Recognizes endogenous human eIF2 alpha only when phosphorylated at Ser51. The antibody does not recognize elF2alpha phosphorylated at other sites. Species crossreactivity: mouse, rat, monkey and D. melanogaster.